To investigate the effect of vermicompost, N fertilizer and their integration on growth, biological and essential yields OIL and its components in two populations (Varamin and Isfahan), an experiment was conducted in a randomized complete block design with three replications in the field of Isfahan Medicinal Plants Research Center in 2016. Fertilizer treatments including 100% urea, vermicompost 33.3 and urea 66.6%, vermicompost 66.6 and urea 33.3%, 100% vermicompost and control treatment (without fertilizer) as the first FACTOR and Varamin and Isfahan populations as the second FACTOR were selected. The results showed that the interaction effects of nitrogen fertilizer and populations on all studied traits were significant. In both populations, nitrogen fertilizer improved height, biological and essential OIL yields, content of essential OIL compounds (d-Carvone and its yield, α-phellandrene and linalool; except p-Cymene and Limonene in Isfahan population). Since in medicinal plants, the quantity (biological yield) and quality (essential OIL and d-Carvone; the most important ingredient and the highest amount of essential OIL in this experiment), 66.6% vermicompost + 33.3% urea in Varamin population, was the best treatment in the experiment. In addition an in the direction of human health and sustainable agriculture, it is possible to reduce 33% of chemical fertilizers application and pollution; however, in terms of other essential OIL contents (α-phellandrene, Linalool and p-Cymene), 100% vermicompost fertilizer treatment was superior in Isfahan population.